Resumo

Rev Cubana Med Trop [online]. 2004, vol.56, n.1, pp.77-79. ISSN 0375-0760.

A comparative study was conducted between the assimilation of D-proline and the growth on canavanine-glycine-bromothymol blue (CGB) medium used for the classification of the varieties of Cryptococcus neoformans. In the 86 studied strains, 100 % of coincidence between both methods allowed to affirm that 95.34 % corresponded to the neoformans var. and the rest (4.65 %) to the. gattii var. The results obtained corroborated that all the autoctonous clinical isolations up to the present correspond to the. neoformans var. and made possible to suggest the use of D-proline for the initial evaluation of strains, as an alternative and simple method that presented under these conditions high coincidence with the reference method (growth in CGB).

Palavras-chave : CRYPTOCOCCOSIS [etiology]; CRYPTOCOCCUS NEOFORMANS [isolation & purification]; CRYPTOCOCCUS NEOFORMANS [classification]; CRYPTOCOCCUS NEOFORMANS [growth and development]; CULTURE MEDIA; CANAVANINE, BROMOTHYMOL BLUE [classification].

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