Abstract

DURAN VILA, Anabel; ZHURBENKO, Raisa  and  VIERA ORAMAS, Diana R.. Proposal of the modified formulation of mannitol salt agar medium used in the isolation of clinically important stafilococci. Rev Cubana Med Trop [online]. 2004, vol.56, n.3, pp.172-177. ISSN 0375-0760.

It was recommended to obtain an optimal product, where the growth speed and the differentiation of the microorganisms in the medium would increase, as a result of the adequate combination of the nutrients. The celerity in the identification and characterization of an infectious agent contribute to apply a suitable therapy to the patient. The Mannitol Salt Agar is a highly selective medium for the isolation of pathogenic staphylococci, that includes the mannitol polihidroxyl alcohol in its composition. This substrate allows the identification of coagulase positive staphylococci belonging mainly to the S. aureus species, due to their capacity to ferment mannitol. This characteristic has been considered as a pathogenicity index. Modifications were carried out in the formulation of the Mannitol Salt Agar, getting a product similar to the reference medium, with superior physical-chemical and microbiological characteristics, and without significant differences (p>0.05) in comparison with the reference product used.

Keywords : Staphylocccus; identification; isolation; culture medium.

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