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Revista Cubana de Medicina Tropical
versión impresa ISSN 0375-0760versión On-line ISSN 1561-3054
Resumen
RODRIGUEZ REYES, Ernesto Armando et al. Expression in Escherichia coli of the gspdl gene of the type II secretion system in Leptospira borgpetersenii serovariety hardjo. Rev Cubana Med Trop [online]. 2005, vol.57, n.1, pp.45-46. ISSN 0375-0760.
A fragment of 1.539 pb of the gene homologous to gspD of Hardjobovis was clonated in the pET28a vector and it was transformed into E. coli C43 and Rosetta strains. The sequence of GspDL showed 46 % of similitude with E. coli GspD secretin. The expression of recombinant GspDL was obtained in Rosetta strain.
Palabras clave : Lepsotspira; secretion system; E. coli.
