Abstract

THEODORIDIS, Dimitrios; BOHMER, Jan; HOMUTH, Matthias  and  STRUTZBERG-MINDE, Katrin. Development of a novel ELISA for serodiagnosis of Leptospirosis and additional detection of pathogenic Leptospira by polymerase chain reaction for veterinary routine diagnostics. Rev Cubana Med Trop [online]. 2005, vol.57, n.1, pp.49-50. ISSN 0375-0760.

A multi-serovar ELISA based on the outer membrane Lipoprotein L41 (LipL41) of pathogenic Leptospira was developed to increase sensitivity by using a single test antigen. A sensitivity of 99 % and a specifity of 92 % could be achieved. The established diagnostic polymerase chain reaction is also able to detect fast and reliably pathogenic Leptospira in different clinical samples.

Keywords : Leptospirosis; leptospira; ELISA; PCR; diagnosis.

        · abstract in Spanish     · text in English     · English ( pdf )