Resumen

GONZALEZ GONZALEZ, Ruby L.; ROQUE QUINTERO, Annele; MORIER DIAZ, Luis  y  RODRIGUEZ LAY, Licel de los Ángeles. Evaluation of the antiviral activity of medicinal plants against the hepatitis B virus (HBV) in PLC/PRF/5 cells. Rev Cubana Med Trop [online]. 2006, vol.58, n.2. ISSN 0375-0760.

The antiviral properties of plants extracts derived from Calendula officinalis L., Psidium guajava L., Eucaliptus spp. y Phyllanthus orbicularis HBK against the hepatitis B virus were evaluated at subtoxic concentrations in the in vitro PLC/PRF/5 system or Alexander cells, a cell line expressing constitutively the virus surface antigen (AgsHB). The cell viability parameter that was measured by the calculation of the mean cytotoxic concentration values (CC50): Eucalyptus spp. showed a lower toxicity in the cells, followed by Psidium guajava L., Phyllanthus orbicularis, and finally Calendula officinalis that had a much higher toxicity than the previous extracts. Later on, it was studied the behaviour of the production of intracellular and extracellular HBsAg of the cells at different concentrations of the extracts during 48 hours of treatment. The data obtained showed an inhibitory activity in the case of Phyllanthus orbicularis, as well as for the eucaliptus extract. With the guava extract, the activity was lower than in the 2 previous cases, whereas calendula did not show any inhibition to the assayed concentrations, which proves the absence of the activity searched in this extract.

Palabras clave : Antivirals; hepatitis B virus (HBV); PLC/PRF/5; medicinal plants.

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