SciELO - Scientific Electronic Library Online

 
vol.58 número2Anticuerpo monoclonal que mimetiza la estructura de un epítope inmunoprotector contra Fasciola hepaticaMeningoencefalitis por Pseudomonas cepacia índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

  • No hay articulos citadosCitado por SciELO

Links relacionados

  • No hay articulos similaresSimilares en SciELO

Compartir


Revista Cubana de Medicina Tropical

versión impresa ISSN 0375-0760versión On-line ISSN 1561-3054

Resumen

GATO ARMAS, René et al. Recognition of intact cells from Cryptococcus neoformans by the anti-glucuronoxylomannan 4B3 monoclonal antibody. Rev Cubana Med Trop [online]. 2006, vol.58, n.2. ISSN 0375-0760.

The capacity of the 4B3 monoclonal antibody, previously obtained by immunization of BALB/c mice with the capsular polysaccharide of Cryptococcus neoformans was studied to recognize this structure as part of the intact cell of this yeast. With this aim, 4B3 was evaluated by cellular ELISA and indirect immunofluorescence, using as an antigen a cell suspension of the 028 LMIPK C. neoformans strain. Serum from the mouse used in the fusion for obtaining 4B3 monoclonal antibody was used as a positive control, whereas an anti-dengue monoclonal antibody was used as a negative control. Both methods demonstrated that the above mentioned monoclonal antibody is capable of recognizing the native antigen, which will allow its future evaluation for diagnostic and therapeutic purposes.

Palabras clave : Cryptococcus neoformans; glucuronoxylomannan; cellular ELISA; immunofluorescence; monoclonal antibody.

        · resumen en Español     · texto en Español     · Español ( pdf )

 

Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons