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vol.59 número1Adaptación de un protocolo de inmunohistoquímica para la detección de Leptospira spp. en muestras de tejido fijado en formaldehídoBrote de leptospirosis humana en la provincia Guantánamo índice de autoresíndice de materiabúsqueda de artículos
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Revista Cubana de Medicina Tropical

versión impresa ISSN 0375-0760versión On-line ISSN 1561-3054

Resumen

Microbiological confirmation of two emergent outbreaks of human leptospirosis in Cuba. Rev Cubana Med Trop [online]. 2007, vol.59, n.1. ISSN 0375-0760.

Clinical samples from 293 patients with suspicion of leptospirosis were studied for the microbiologic confirmation of these events, in October-December 2005, when there were 2 outbreaks in humans, in Cuba. Sera samples of patients in acute phase and during convalescence, as well as hemocultures performed before the beginning of the antibiotic therapy, were analyzed. Conventional techniques (passive hemagglutination test and serogroups hemagglutination), and advanced or fast diagnosis techniques (Lepto tek Dri-Dot, Lepto-Cuba, Latex-India, Lepto tek Lateral Flow) were used for serologic diagnosis. In outbreak I, 26 % of the studied cases were confirmed by serological tests (22/84), and 25 % (5/20) through hemocultures; whereas in outbreak II, 48 of the 162 studied cases (30 %) were serologically confirmed, and it was possible to obtain isolation of leptospires in 6 of the 27 processed cases (22 %). The main serovariants found by serology were canicola, ballum, icterohaemorrhagiae, and pomona. The rapid diagnosis methods were useful screening tools in the most severe cases or at pediatric ages. The two epidemiologic events were caused by pathogenic leptospires infection, which contributed to the adoption of hygienic-sanitary measures in both provinces.

Palabras clave : Leptospirosis; epidemiologic outbreaks; diagnosis.

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