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Revista Cubana de Medicina Tropical

Print version ISSN 0375-0760On-line version ISSN 1561-3054

Abstract

DIAZ PANTOJA, Cristina; ALVAREZ GAVILAN, Yudelmis; DE ARMAS RODRIGUEZ, Yaxsier  and  BISSET LAZCANO, Juan A.. Determination of insecticide-resistance and resistance mechanisms of Blattella germanica (Dictyoptera: Blattellidae). Rev Cubana Med Trop [online]. 2007, vol.59, n.2. ISSN 0375-0760.

In this paper, the level of resistance to four insecticides of 3 Blatella germanica strains collected from various places in the City of Havana province was evaluated. These strains were resistant to two pyrethroids (cypermethrin and lambdacyalothrine) and to organophosphorate malathion but susceptible to carbamate propoxur. The values of α and β esterases, acetylcholinesterase and gluthatione-S-transferase were estimated in three strains involved in the study. The results of the study showed high esterase activity in all the strains, mainly β esterases and two of the three strains presented with high gluthation-S-transferase enzyme. No changes in acetylcholinesterase were demonstrated in relation to the reference strain. The association of levels of resistance to insecticides, the possible resistance mechanisms in each strain and the results of the enzymatic activity were also analyzed.

Keywords : Blatella germanica; mechanisms; resistance; insecticides; micromethods.

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