My SciELO
Services on Demand
Journal
Article
Indicators
- Cited by SciELO
Related links
- Similars in SciELO
Share
Revista Cubana de Medicina Tropical
Print version ISSN 0375-0760On-line version ISSN 1561-3054
Abstract
MARTIN ALFONSO, Dayamí et al. Design and evaluation of DAVIH VIH-2 . Rev Cubana Med Trop [online]. 2007, vol.59, n.3. ISSN 0375-0760.
The results of the design and evaluation of DAVIH VIH-2 diagnosing system, an indirect Elisa for screening of HIV-2 antibodies, which uses a HIV-2 glycoprotein gp36 synthetic peptide in its solid phase, were exposed. In the system evaluation using WHO reference panels, 100 % sensitivity, 99,81 % specificity, 99,81% efficacy and very good concordance level (kappa = 0.978) were attained. Serum samples of 959 individuals with undetermined or negative results to the HIV-1 antibodies confirmation (DAVIH blot) were evaluated by the DAVIH VIH-2 system. Twenty four samples were reactive, six of which had confirmed HIV-2 antibodies. These results allowed recommending the introduction of this diagnostic kit in the HIV infection diagnosing algorithm in Cuba.
Keywords : HIV-2 antibodies; AIDS serodiagnosis; validation studies; ELISA /methods.