Resumen

PEREZ, Ana B. et al. TNFa and nitric oxide ex vivo production by blood cells due to dengue virus. Rev Cubana Med Trop [online]. 2008, vol.60, n.1. ISSN 0375-0760.

OBJECTIVE: High TNFa levels have been associated with the development of severe forms of dengue virus infection. The NO production seems to be lower in dengue hemorrhagic fever cases compared to dengue fever cases. However, the role of those mediators in the tertiary dengue infection has not been discovered yet. METHODS: The ability of whole blood cells from individuals with a history of dengue infection of producing TNFa and Nitric oxide (NO) when cultured with a different serotype of dengue virus mimicking an ex vivo tertiary infection, was measured in the culture supernatants by using a standard ELISA kit and Griess test, respectively. RESULTS: Dengue virus stimulation induced the release of very high and variable TNFa levels in whole blood cells supernatant, thus supporting the possible role of this cytokine in the pathogenesis of a symptomatic tertiary dengue infection. In these experimental conditions, there was no difference in the NO synthesis between DF and DHF cases, showing an apparent inhibition due to heterotypic antibodies. CONCLUSIONS: The dispersion of TNFa levels among individuals could have some effects on the development of a tertiary natural dengue infection.

Palabras clave : Dengue; TNFa; nitric oxide.

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