Abstract

FRAGAS QUINTERO, Anitza et al. Preliminary results of DAVIH-AgP24 with the biotin-tiramyne/streptavidine-peroxidase amplification system. Rev Cubana Med Trop [online]. 2009, vol.61, n.1. ISSN 0375-0760.

OBJECTIVE: ELISA DAVIH-Ag p24 was modified by introducing heat dissociation of plasma samples and a tyramine/streptavidine-peroxidase amplification system, with the objective of increasing sensitivity. METHODS: Between-assay repeatability was determined in amplified DAVIH-Ag p24. Thirty two plasma samples from HIV-1-infected individuals classified in three clinical categories (AIDS case, asymptomatic and minor opportunistic infections) were evaluated. RESULTS: the variation coefficient ranged 4-10.3 % in between-assay repeatability. With the amplified DAVIH-p24 Ag, the p24 antigen detection level increased to 0.5 pg/mL. Amplified DAVIH-p24 Ag reached 66 % sensitivity whereas standard DAVIH-p24 Ag sensitivity rate was 31 %. CONCLUSIONS: This preliminary study proved that the introduction of new modifications in amplified DAVIH-p24 Ag managed to increase the p24 antigen detection levels and to gain sensitivity.

Keywords : p24 antigen; immune complex dissociation; biotin-tyramine/streptavidine-peroxidase amplification system; antiretroviral therapy.

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