Revista Cubana de Medicina Tropical
On-line version ISSN 1561-3054
MACHADO ZALDIVAR, Liuber Y.; BLANCO DE ARMAS, Madeline; LUBIAN CABALLERO, Ana Luisa and DIAZ TORRES, Héctor M.. Use of Amplicor HIV-1 DNA test v.1.5 in diagnosis of perinatal infection from HIV-1 in Cuba. Rev Cubana Med Trop [online]. 2010, vol.62, n.2, pp. 154-156. ISSN 1561-3054.
INTRODUCTION: HIV-1 proviral DNA amplification is the preferential method for HIV diagnosis infection in infants and it has been used in Cuba since 1992. AMPLICOR HIV-1 DNA kit is an in vitro qualitative assay for the detection of HIV-1 proviral DNA in the whole blood. OBJECTIVE: this paper showed the results of the use of this kit for the first time in Cuba for the perinatal diagnosis of HIV-1 infection. METHODS: three hundred forty six whole blood samples from children of HIV seropositive women were analyzed by the AMPLICOR HIV-1 DNA kit in the period 2005-2007. RESULTS: among the tested samples, six were positive, and 340 negative. CONCLUSIONS: the assay was reproducible under the Cuban conditions and the achieved results made the diagnosis and follow up of children of HIV-1 seropositive mothers possible.
Keywords : HIV-1; molecular diagnosis; mother-to-child trasmission prevention; polymerase chain reaction.