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Revista Cubana de Medicina Tropical
versão On-line ISSN 1561-3054
Resumo
OTERO GONZALEZ, Anselmo J. Enzymatic immunoassays for the detection of infectious agents or their products: presentation of some designs and applications. Rev Cubana Med Trop [online]. 2010, vol.62, n.3, pp. 167-179. ISSN 1561-3054.
This paper assessed the impact of the immunoenzymatic assays on the field of the immunochemistry-based analytics for the last 40 years, and on the detection of infectious agents or the products related to their presence and/or pathogenic activity. It also addressed some designs and formats of these immunoassays from electrochemical methods of detection, assays to determine proteolytic microbial activity and their inhibitors as possible therapeutical targets, more sensitive direct triple antibody systems, alternative enzymatic activity detectors, assays for viral serology of minimal determinations to competitive assays for evaluation of vaccinal candidate effectiveness based on selected peptide combinations. Finally, it provided a rapid overview of the near future of this type of immunoassays in the light of the emerging detection analytical technologies.
Palavras-chave : antigen-antibody reaction; specificity; immunoassays; infectious agents; immunodetection.