Resumen

GONZALEZ SAMPEDRO, Renée et al. Description of an enzime immunoassay that recognizes anti-factor VIII/von Willebrand antibodies obtained from murine hybridomas. Rev Cubana Hematol Inmunol Hemoter [online]. 1999, vol.15, n.1, pp.46-53. ISSN 0864-0289.

An enzime immunoassay (ELISA) that detects anti-Factor VIII/von Willebrand (FVIII/vw) antibodies with a binding relationship > 5 that allows the early recognition of such antibodies in the cultures of murine hybridomas is described. Semipurified preparations of FVIII/vw obtained in Cuba were used for the adsorption of the immunoassay plates. Balb/c mice were immunized in order to generate the monoclonal antibodies detected by this ELISA. A clone with stable growth and several positive subclones were obtained by ELISA with specific biological activity of FVIII-inhibiting antibodies

Palabras clave : VON WILLEBRAND FACTOR; HYBRIDOMAS; ANTIBODIES; ENZYME-LINKED IMMUNOSORBENT.

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