Revista Cubana de Hematología, Inmunología y Hemoterapia
versão On-line ISSN 1561-2996
LEVON HERRERA, Raquel et al. Molecular study of MLL gen in 30 patients with acute leukemias. Rev Cubana Hematol Inmunol Hemoter [online]. 2000, vol.16, n.1, pp. 38-44. ISSN 1561-2996.
Rearrangements of MLL gen in llq23 chromosomal band are frequents in childhood type of acute leukemia (AL) and in secondary AL, developed after therapy with II topoisomerase enzyme. To a lesser extent also is seen in adults with AL. Presence of theses rearrangements is considered to be a worse prognosis indicator, associated with unfavourable clinical results, that is why it is very important to carry our its assessment in AL. In this paper authors present preliminary results from introduction of study on MLL gen in our country through Southern technique. DNA from 30 patients was analized, including children and adults, that at the very moment when study was performed, they were in the onset or in relapse. Molecular study was carried out using FA4 probe, which is a genomic insertion of MLL gen. Only one out of 30 patients, presented rearrangement band with 2 distinct restriction enzymes, remainder showed MLL gen in germinal form. It is interesting to underline that patient with rearrangement was a child presenting subtype M5b acute myeloblastic leukemia, which agree with literature showing that these rearrangements are closely correlated to subtype myelomonocytic (M4) and monocytic (M5) of acute myeloid leukemia (AML)
Palavras-chave : GENE REARRANGEMENT; HEMATOLOGIC DISEASES; LEUKEMIA, LYMPHOCYTIC.