Resumen

PEREZ TOLEDO, Liliana et al. Assessment of recognition ability of conjugate monoclonal antibodies: IOR T3, T4 and T8. Rev Cubana Hematol Inmunol Hemoter [online]. 2000, vol.16, n.1, pp. 62-66. ISSN 1561-2996.

In VIH infection, sequential assessment of CD3, CD4, and CD8 lymphocytes values, provide information to stablishment of clinical status and prognosis of patients. Availability of optimal reagents of national manufacture to use in flow cytometry, means a saving without to affect quality. Diagnostic Laboratory of "Pedro Kourí" Institute of Tropical Medicine (IPK) to correlates percentage of lymphocytes recognized by conjugate monoclonal antibodies (MAB), produced by Center of Molecular Immunology (CMI) (CIMAB S.A. Havana, Cuba) [ior T3, T4, and T8] versus homologous of DAKO AG commercial firm (Denmark) in randomized patient´s samples in different diseases stage. Results of correlation coefficient to each MAB, using single regression were as follow: CD3=0,932; CD4=0,986; CD8=0,958. This study corroborates that conjugated MAB of ior serie (CIMAB S.A.), behaves as for recognition percentage, in similar way to that of DAKO AG commercial firm, making possible diagnosis and monitoring of HIV/AIDS Cuban patients

Palabras clave : FLOW CYTOMETRY; LABORATORY TECHNIQUES AND PROCEDURES; HIV INFECTIONS.

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