Resumen

ALFONSO VALDES , Yalile  y  BENCOMO HERNANDEZ, Antonio. Procedures for the detection and identification of red cell antibodies. Rev Cubana Hematol Inmunol Hemoter [online]. 2001, vol.17, n.2, pp.98-107. ISSN 0864-0289.

Awide range of procedures for the detection and identification of red cell antibodies in vitro has been developed in Immunohematology. Therefore, it is made a review of the techniques and methods used with this aim, such as the method using erythrocytes pretreated with proteolytic enzimes and the techniques of Polibreno that utilize low ionic force solution (LIFS), the indirect antiglobulin test, the gel agglutination test, the agglutination inhibition test, hemolysis and the solid phase erythrocyte adherence test. The problems affecting the agglutination reaction between the antigen and the antibody are also dealt with. The agglutination reaction is subdivided into its first and second phase for a better understanding. In the first phase, the antigen and antibody concentration, pH, temperature, ionic force and incubation time are analyzed. The characteristic of the antibody, localization and number of antigenic sites, forces maintaining the distance among the erythrocytes, use of bovine albumin, use of enzimes, dose effect and effect of molecules with positive charge are studied in the second phase

Palabras clave : ANTIGEN-ANTIBODY REACTIONS [immunology]; ERYTHROCYTES; BINDING SITES, ANTIBODY; IMMUNOLOGIC TECHNIQUES.

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