Revista Cubana de Hematología, Inmunología y Hemoterapia
versão On-line ISSN 1561-2996
ARCE HERNANDEZ, Ada A et al. Purification of IgG1, IgG2 and IgG3 by affinity chromatography starting from Intacglobin. Rev Cubana Hematol Inmunol Hemoter [online]. 2001, vol.17, n.2, pp. 138-141. ISSN 1561-2996.
A method for the purification of IgG1, IgG2, and IgG3 subclasses starting from Intacglobin by affinity chromatography with the use of protein A Sepharose and the application of a lineal gradient of pH is described. IgG1, IgG2 and IgG3 were detected in the eluted fractions by doble immunodifussion and immunoelectrophoresis by using anti IgG, anti IgG1, anti IgG2 and anti IgG3 commercial sera. The procedure developed was characterized by its simplicity, high resolution and the relative purity of the isolated subclasses. A total yield of 53.7 % was attained, which demonstrates the efficiency of this method
Palavras-chave : IGG [isolation purification]; CHROMATOGRAPHY, AFFINITY [methods]; IMMUNE SERA.