Resumo

DEL VALLE PEREZ, Lázaro et al. Effect in vitro of hemin on the proliferation of human lymphocytes. Rev Cubana Hematol Inmunol Hemoter [online]. 2002, vol.18, n.2. ISSN 0864-0289.

The effect in vitro of hemin on human lymphocytes was studied. Thirty donors from the blood bank of the Institute of Hematology and Immunology were selected, who were performed tests of active rosette, of blastic transformation with tritiated thymidine and the expression of HLA-DR and CD-25 activation antigens by immunocytochemical ultramicromethod with and without hemin. Statistically significant differences under experimental conditions with and without hemin were found. It was confirmed that hemin by itself is capable of promoting blastic transformation, increasing the formation of active rosette and the expression of HLA-DR and CD25 activation antigens. In the literature review, no results similar to the above-mentioned were found. Hemin is a product that can activate human lymphocytes by inducing their proliferation and therefore, the expression of activation antigens. The paper suggests that preclinical and clinical studies should be carried out to evaluate hemin as a probable drug for the treatment of cell immunodeficiencies

Palavras-chave : HEMIN [isolation / purification]; LYMPHOCYTES; HLA-DR ANTIGENS; ROSETTE FORMATION; BLOOD DONORS; LYMPHOCYTE TRANSFORMATION.