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Revista Cubana de Hematología, Inmunología y Hemoterapia

versión impresa ISSN 0864-0289versión On-line ISSN 1561-2996

Resumen

DEL VALLE PEREZ, Lázaro O et al. Effect in vitro of Calendula Officinalis L: extract on human lymphocytes. Rev Cubana Hematol Inmunol Hemoter [online]. 2002, vol.18, n.3. ISSN 0864-0289.

Calendula Officinalis L is an ornamental and medicinal plant to which many immunoestimulating properties are attributed by the traditional medicine. The effect in vitro of an extract from this plant(100 mg/ML) on human lymphocytes from 30 healthy donors and 30 patients diagnosed with cellular immunodeficiency was studied through lymphoblastic transformation following tritated thymidine and active rosette criteria. No statistically significant differences were found under experimental conditions without and with Calendula officinalis L. dilutions (from 1:2 to 1:2048) in blastic transformation test. As to active rosette test, there was no difference between lymphocytes without and with incubated extract (dilution rate: 1:64) during the 24 hours before the rosette formation. It is concluded that Calendula officinalis L extract in vitro does not bring about changes in human lymphocytes from both healthy donors and patients diagnosed with cellular immunodeficiency

Palabras clave : CALENDULA; LYMPHOCYTE TRANSFORMATION; ROSETTE FORMATION; IN VITRO.

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