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Revista Cubana de Hematología, Inmunología y Hemoterapia
versão impressa ISSN 0864-0289versão On-line ISSN 1561-2996
Resumo
ARCE HERNANDEZ, Ada Amalia; VILLAESCUSA BLANCO, Rinaldo e MERLIN LINARES, Julio César. Purification of band 3 protein from human erythrocytes (AE 1). Rev Cubana Hematol Inmunol Hemoter [online]. 2007, vol.23, n.1. ISSN 0864-0289.
The band 3 protein is purified starting form the membranes of human erythrocytes with celerity and efficiency by a combination of DEAE-cellulose ion-exchange and affinity chromatography by using a pCMB-Sepharose column. The obtention of band 3 will allow to investigate its possible participation in the occlusive phenomena in drepanocytosis
Palavras-chave : band 3; chromatography; DEAE-cellulose; drepanocytosis; human erythrocyte membrane; pCMB-Sepharose 4B.