Resumo

ARCE HERNANDEZ, Ada Amalia; VILLAESCUSA BLANCO, Rinaldo  e  MERLIN LINARES, Julio César. Immunoenzimatic assay to determine the natural anti-band 3 antibodies. Rev Cubana Hematol Inmunol Hemoter [online]. 2008, vol.24, n.3. ISSN 0864-0289.

An immunoenzimatic assay (ELISA) was standardized to determine the natural anti-band 3 antibodies by coupling with Chemobond plates the band 3 protein obtained from the membrane of human erythrocytes by a combination of affinity and anion exchange chromatography. 3 g of band 3 protein per well were used. A level of detection of 1 g/mL of anti-band 3 antibody was achieved. The results showed the usefulness of the method to measure the levels of natural anti-band 3 antibodies in patients with drepanocytes, both in basal state and in the painful vasocclusive crises.

Palavras-chave : natural anti-band 3 antibodies; drepanocytosis; ELISA; band 3 protein.