Abstract

BENCOMO-HERNANDEZ, Antonio A. et al. Relation between hemolysis with presence and quantification of immunoglobulin in red blood cells in cases of autoimmune hemolytic anemia. Rev Cubana Hematol Inmunol Hemoter [online]. 2010, vol.26, n.4, pp.315-327. ISSN 0864-0289.

A group of 135 patients was studied: 111 adults and 24 children presenting hot autoimmune hemolytic anemia (HAIHA) The erythrocyte characterization and the number of immunoglobulin molecules by red blood cells was determined by ELISA. In 51 patients the pattern of IgG subclasses was investigated. The hemolysis intensity was classified by the hemoglobin concentration, the reticulocytes count and the figures of plasma haptoglobin. In patients presenting with high degree hemolysis IgM autoantibodies were detected in combination with IgG, IgA isotypes. In cases only with the presence of IgG, the number of molecules of autoantibodies by red blood cells was the determinant factor in hemolysis. There were not differences in relation to subclasses pattern. The severity of hemolysis in HAIHA is related to the coexistence of multiple immunoglobulins in red blood cells and especially of the IgM.

Keywords : Autoimmune hemolytic anemia; autoantibodies; IgG subclasses; quantification; ELISA.

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