Revista Cubana de Hematología, Inmunología y Hemoterapia
versão ISSN 0864-0289
GONZALEZ IGLESIAS, Ana Iris et al. Obtaining and processing of peripheral blood hematopoietic progenitor cells for cell therapy of angiological diseases. Rev Cubana Hematol Inmunol Hemoter [online]. 2011, vol.27, n.3, pp. 356-364. ISSN 0864-0289.
The safety and effectiveness of a manual collection method of peripheral blood hematopoietic progenitor cells mobilized by the Cuban-made granulocytic colony-stimulating factors (Leukocim and Hebervital) were evaluated. Two hundred patients, who had been selected for the cell therapy at the Angiology Service of "Enrique Cabrera" General Teaching Hospital, were studied. The method designed by the Institute of Hematology and Immunology served to obtain and separate autologous mononuclear cells from the peripheral blood. For the purpose of assessing the efficacy of this method, the variables contents of nucleate cells, of mononucleate cells and of CD 34+ cells were analyzed in the final concentrate. Additionally, the cell viability and the microbiological pollution were determined. The efficiency and safety of the collecting and processing method for obtaining one concentrate with adequate content of mononuclear cells and no significant clinical complications was confirmed. The efficacy of the Cuban granulocytic colony-stimulating factors was proved. The adverse effects of the mobilization were mild and unrelated to the used stimulating factor.
Palavras-chave : regenerative medicine; stem cells; granulocytic colony-stimulating factors; mononuclear cells.