Resumo

REMIGIO MONTERO, Antonia; GONZALEZ CARVAJAL, Yousy; ZAMORA RODRIGUEZ, Zullyt  e  FONSECA LOPEZ, Gladys. ¿Influye la ozonoterapia sobre la médula ósea de ratones tratados?. Rev Cubana Invest Bioméd [online]. 1999, vol.18, n.1, pp.24-26. ISSN 0864-0300.

The genotoxic activity of oleozone was studied by the bone marrow micronucleus test in mice of the Cenp:NMRI line of both sexes. This assay is the indirect determination of the induction of chromosomal structural or numerical aberrations. The genotoxic evaluation of oleozon was carried out through the analysis and comparison of the frequencies of micronuclei in polychromatic erythrocytes in bone marrow obtained for the treatment with ozonized sunflower oil with index of peroxides between 617 and 680 and with the controls sunflower oil and cyclophosphamide (40 mg/kg of body weight), a substance with clastogenic properties that produces an increase in the chromosome breakages. Five consecutive intragastric doses were administered with intervals of 24 hours from a corresponding dose to the maximum doses (2 g/kg/day) based on the absence of toxicity in the oral subchronic studies of the product. No toxic effects were observed in the erythrocytic population studied. Negative results were obtained in the induction of clastogenecity (induction of chromosome aberrations) on quantifying the number of micronuclei present in polychromatic erythrocytes for the index of peroxide studied.

Palavras-chave : OZONE [toxicity]; BONE MARROW [drug effects]; ERYTHROCYTES [drug effects]; MICRONUCLEUS TESTS; MICE.

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