Resumo

GONZALEZ RAMIREZ, Victoria Esther; GONZALEZ GRIEGO, Antonio; RAMIREZ ALBAJES, Victoria  e  ALERM GONZALEZ, Alina. Inmunogenicidad de la vacuna cubana recombinante heberbiovac HB en modelos experimentales y aplicados al humano. Rev Cubana Invest Bioméd [online]. 2000, vol.19, n.1, pp.34-44. ISSN 0864-0300.

One of the fundamental pillars in the control, elimination and possible eradication of B-D viral hepatitis is the use of the Cuban-made hepatitis B vaccine. Experimental models as well as models for humans were developed to study immunogenicity using various routes of administration, doses and schedules. Balb/c mice were used on experimental basis to which 1 mg/dose were administered by intramuscular(IM) and intraperitoneal (IP) within three different vaccination schedules, i,e, at 0, 1, and 2 weeks; 0, 1 and 4 weeks, and 0, 4 and 8 weeks. Immunogenicity was stufied through quantification of antibodies to surface antigens by using an immunoenzymatic method. Seroconversion was present in all animals at 0, 1 and 4 and 0, 1, and 8 weeks schedule. However, in 0, 1 and 2 schedule, IP route of administration was more immunogenic than IM, with 60% and 35% seroconversion respectively. IP route had a lower variation coefficient than 0, 1 and 4 week and 0, 4 and 8 schedules were compared, but anyway, it was high even for Balb/c animals. Medical and nursing students were immunized with 20 mg/dose IM and 2mg/dose intradermally within 0, 1 and 4 week and 0,4 and 8 w schedules. It was showed that seroprotection and hyperesponse were greater in 0, 4 and 8 week vaccination schemes regardless of the route of administration and that 0, 1 and 4 w schedule does not bring required immunogenicity, therefore, it should be used exceptionally. National antibody standards were obtained to be applied in anti-HbsAg quantification methods both in experimental and human-based studies

Palavras-chave : HEPATITIS B VACCINES [immunology]; VACCINES, SYNTHETIC; MICE INBRED BALBc; STUDENTS, NURSING; STUDENTS; MEDICAL; CUBA.

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