Revista Cubana de Investigaciones Biomédicas
On-line version ISSN 1561-3011
CESPEDES, Ela et al. Caracterización de indicadores bioquímicos de estrés oxidativo en el asma bronquial. Rev Cubana Invest Bioméd [online]. 2000, vol.19, n.3, pp. 159-163. ISSN 1561-3011.
120 patients with diagnosis of bronchial asthma that were attended at the Service of Pneumology of "Hermanos Ameijeiras" and "Calixto Garcia" hospitals were studied. They were grouped according to the frequence of appearance of the crisis into patients in intercrises: those with absence of signs and symptoms for more than 2 months, and patients in crisis: those who had symptoms and signs. The clinical criteria used to refer to the severity of the disease were: severe asthma, moderate asthma and mild asthma. The control group was composed of apparently sound individuals who were blood donors at the Provincial Blood Bank. The determination of malondialdehyde, a product of lipid peroxidation was used as an expression of the oxidative damage to the pulmonary parenchima. The concentration of malondialdehyde proved to be higher among patients in crisis. No significant differences were observed according to the severity of the disease. Phospholipase A2 played an important role in the rechange of membranes and exocitosis. The activity of phospolipase A2 was higher among patients with severe crisis than in the control group. In the study, it was found a remarkable reduction of the superoxide dismutase activity during the crisis that was lower in mild and moderate asthma. It seems that in spite of the fact that the enzime activity decreases in the asthmatic patients in general, there is an overexpression during the exacerbation of the disease. There were no significant differences in the catalase activity
Keywords : OXIDATIVE STRESS; ASTHMA [drug therapy]; ASTHMA [enzymology]; LIPID PEROXIDATION; SUPEROXIDE DISMUTASE [analysis]; CATALASE [analysis]; ANTIOXIDANTS [therapeutic use]; PHOSPHOLIPASES A [analysis]; MALONDIALDEHYDE [analysis].