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Revista Cubana de Investigaciones Biomédicas

versão impressa ISSN 0864-0300versão On-line ISSN 1561-3011

Resumo

LOPEZ MATILLA, Lien; GONZALEZ GRIEGO, Antonio; SANTIESTEBAN TORRES, Jorge Arturo  e  ALERM GONZALEZ, Alina. Rediseño y estandarización de un método inmunoenzimático para detectar anticuerpos anti-estreptoquinasa en muestras de suero humano. Rev Cubana Invest Bioméd [online]. 2002, vol.21, n.2, pp.69-75. ISSN 0864-0300.

A sandwich, heterogenous, qualitative, doble antigen immunoenzimatic method was standardized to detect anti-streptokinase antibodies in pure samples of human serum. Different concentrations of the recovering antigen and conjugate dilutions, as well as various times and temperatures of incubation of the samples were assayed. The cut-off value of the assay and its detection limit were established and it was proved if there were some unspecificities. 0.1 mg/mL was determined as an optimal recovering concentration for a conjugate dilution of 1:100 000. The optimum conditions of incubation of the samples were 30 min at lab temperature and there were no interferences of other analytes with the technique.The optical densities between 0.282 and 0.654 were established as normal values of the technique.

Palavras-chave : STREPTOKINASE [analysis]; ENZYME-LINKED IMMUNOSORBENT ASSAY [standards]; STREPTOCOCCUS; STREPTOCOCCAL INFECTIONS [diagnosis]; SEROLOGIC TESTS.

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