Revista Cubana de Investigaciones Biomédicas
versão On-line ISSN 1561-3011
LOPEZ MATILLA, Liudmila et al. Desarrollo de un modelo de aterosclerosis inducida por inmunocomplejos de forma activa en ratones BALB/CBJ. Rev Cubana Invest Bioméd [online]. 2002, vol.21, n.2, pp. 91-97. ISSN 1561-3011.
6-week-old BALB/CBJ isogenic mice were used in this study and an ad libitum regime of water and food was established. These mice were divided into 2 groups.One of them was applied the immunization scheme of 0-1-4 weeks with the Cuban anti-hepatitis vaccine by intraperitoneal route. The control group was immunized by the same way, using the excipient of the vaccine without the hepatitis B surface antigen (AgsHB). The treated group inhaled cigarrete smoke daily during a month. After quantifying the anti-hepatitis B antibodies and proving their absence in the controls, 7 immunizations were performed with an interval of 5 days between them by the caudal vein. Recombinant AgsHB was used as an immunogen in the treated group at a dose that dobled the concentration of anti-hepatitis-B antibodies of every animal. The control group was adminstered a saline solution with the same methodology than the treated group. It was determined the presence of circulating immunocomplexes in plasma. Euthanasia was practiced and the presence of atherosclerotic injuries was proved in a macroscopic and microscopic way. According to the results obtained, it was concluded that it was possible to obtain a model of atherosclerosis in BALB/CBJ isogenic mice by the formation of immunocomplexes in an active way.
Palavras-chave : ATHEROSCLEROSIS [immunology]; MICE, INBRED BALBC; DISEASE MODELS, ANIMAL; HEPATITIS B SURFACE ANTIGENS; ANTIGEN-ANTIBODY COMPLEX.