Revista Cubana de Investigaciones Biomédicas
On-line version ISSN 1561-3011
HIDALGO RODRIGUEZ, Roxana; CHIROLES DESPAIGNE, Sonia and VILLAVICENCIO BETANCOURT, Odalys. Quantitative evaluation of the efficiency of a low temperature steam chemical sterilizer using formaldehyde 2 %. Rev Cubana Invest Bioméd [online]. 2006, vol.25, n.1, pp. 0-0. ISSN 1561-3011.
A method of microbiological analysis was developed to evaluate the efficiency of Matachana sterilizer 130 LF made in Spain, whose functioning principle is based on the use of low temperatures, using formaldehyde 2 % at steam stage as a sterilizing agent. The evaluations performed to this technology were compared with the reference method of chemical sterilization that uses a mixture of CO2 with ethylene oxide. Fragments of catheters chemically composed of polyvinyl chloride and fluoroethylene propylene or teflon, which were previously innoculated with reference bacterial strains producing endospores (Bacillus stearothermophilus and Bacillus subtilis) suspended in culture media with addition of organic and inorganic matter, were used to assess the diffusion process of the sterilizing agent in a restricted environment. The result of the evaluations was satisfactory. 100 % of sterility was attained at each cycle under the stated conditions.
Keywords : Efficiency; formaldehyde 2 %; sterilization at low temperatures.