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Revista Cubana de Investigaciones Biomédicas

versión impresa ISSN 0864-0300


HERNANDEZ BETANCOURT, Oscar et al. Stability of a multienzyme control serum used as reference material in clinical laboratories. Rev Cubana Invest Bioméd [online]. 2012, vol.31, n.2. ISSN 0864-0300.

A fundamental method to assure the quality of clinical laboratory tests is the use of reference materials. A problem we are faced with is the scarcity of these products in the domestic market, due their high cost. Objective: Evaluate the stability of an adult bovine serum enriched with the enzymes alanine aminotransferase (ALT, GPT), aspartate aminotransferase (AST, GPT), alkaline phosphatase (AP) and amylase. Methods: This enzyme-enriched matrix underwent real-time stability assessment during 12 months at two temperatures (refrigerated and frozen). An evaluation was made of the effect of glycerol on the enzymatic activity of extracts, as well as the effect of the preservatives propylene glycol and ethylene glycol on enzymatic stability. Results: The enzyme extracts obtained began to lose their biological activity at 15 days, irrespective of the storage temperature and the presence or absence of glycerol. The real time assessment of the enriched matrix showed that stability varied with time and enzyme type, irrespective of the preservative tested, and fell below acceptable limits of enzymatic activity after three months of storage at 4 ºC. Conclusions: A multienzyme reference material was obtained which was stable for a period of 3 months.

Palabras clave : enzymes; ALT; AST; AP; amylase; control serum; glycerol; enzymatic stability; reference material.

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