SciELO - Scientific Electronic Library Online

 
vol.39 número1Acciones para la preparación pedagógica de los alumnos ayudante en ciencias básicas biomédicasEstudio de la precisión de un localizador de ápice electrónico en dientes monorradiculares índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

  • Não possue artigos citadosCitado por SciELO

Links relacionados

  • Não possue artigos similaresSimilares em SciELO

Compartilhar


Revista Cubana de Investigaciones Biomédicas

versão impressa ISSN 0864-0300versão On-line ISSN 1561-3011

Resumo

DE LA PUENTE LOPEZ, Vivian; CUTINO JIMENEZ, Ania M.  e  LOPEZ GONZALEZ, Tania. Molecular markers for the taxonomy and identification of the genus Brucella (Alphaproteobacteria). Rev Cubana Invest Bioméd [online]. 2020, vol.39, n.1, e336.  Epub 01-Mar-2020. ISSN 0864-0300.

Introduction:

Brucella is a genus from the Brucellaceae family, Rhizobiales order. This genus is recognized for its high pathogenicity. Brucella bacteria cause brucellosis, a disease reported as one of the most important zoonoses worldwide due to its incidence in cattle and people. Previous studies on taxonomic classification of the genus have been mainly based on the analysis of gene 16S rDNA. However, few studies have been aimed at identification of molecular markers distinguishing its members from other groups of bacteria.

Objective:

Identify insertions in preserved protein sequences which could be used as molecular markers for the taxonomy and diagnosis of species from the Brucella genus.

Methods:

The homologous sequences for the proteins analyzed were obtained from international databases and aligned with the software ClustalX2, considering the parameters suggested in the literature.

Results:

Insertions were identified in the proteins oxoglutarate dehydrogenase (component E1) and DNA ligase A, specific of the genus Brucella.

Conclusions:

The insertions found may be used as complements to the traditional methods for taxonomic classification and for the molecular diagnosis of bacteria from the genus Brucella.

Palavras-chave : Brucella; molecular markers; insertions; oxoglutarate dehydrogenase; DNA ligase A.

        · resumo em Espanhol     · texto em Espanhol     · Espanhol ( pdf )