Pastos y Forrajes
versão ISSN 0864-0394
BECQUER, C.J et al. Selection of rhizobium strains isolated from livestock ecosystems of Canada, inoculated to wheat (Triticum aestivum L.). Pastos y Forrajes [online]. 2007, vol.30, n.2, pp. 1-1. ISSN 0864-0394.
A field experiment was carried out in order to select rhizobial strains inoculated to wheat (Triticum aestivum, L., var. Cuba-204). Several parameters of agricultural significance were assessed, such as: aerial dry weight, length of stems, grain yield, weight of 1 000 grains and nitrogen yield. As selection criterion of the best treatments, the statistically higher values compared to the fertilized control were considered. Four reference strains, belonging to several rhizobial genus and species were used, as well as ten native strains, belonging to Sinorhizobium that were isolated from roots of legumes (Melilotus and Medicago), adapted to Canadian livestock ecosystems in Alberta. One of the strains was isolated from legumes adapted to soils contaminated with hydrocarbons located in the same geographic zone. The strains grew YMA and re-suspended in YMB until they reached a 6 8 CFU of 10 - 10 cells/mL. Standard methods for the inoculation of cereals were used. A complete randomized block experimental design with 16 treatments and four replications within plots was used. A treatment was fertilized with 150 kg/ha (NH NO ). Variance analysis was applied while differences between treatments were 4 3 found through LSD (Fisher’s test) (p<0, 05). A dendrogram was built through the centroid method (Euclidian distance). It was concluded that a significant increase of values in all the variables was shown as compared to the absolute control. Several treatments, inoculated with reference or native strains showed values statistically higher than those of the fertilized control. Six rhizobial strains of highly promising use for the local agriculture were selected. Biofertilization of wheat with rhizobia is strongly recommended as an alternative of high ecological and economic value for the agriculture of Cuba.
Palavras-chave : Inoculation; Sinorhizobium; Triticum aestivum.