Abstract

FUENTES, Leticia et al. Influences of the NaCl in biochemical indicators evaluated in callus of Stylosanthes guianensis CIAT-184. Pastos y Forrajes [online]. 2008, vol.31, n.1, pp.1-1. ISSN 0864-0394.

The accumulation of certain metabolites and the changes in the activity of antioxidant enzymes, such as catalases and peroxidases, can be related to the plant possibilities for counteracting the negative effects of salinity on physiological and morphological processes. In this work, the levels of total proteins and carbohydrates, and the enzymatic activities of catalases and peroxidases were determined in calluses obtained from three explants of Stylosanthes guianensis CIAT-184, cultured in MS medium supplied with 1 mg/L of 2,4-D, 2 mg/L de 6 BAP and different concentrations of NaCl (0, 25, 50, 75 and 100 mM). Although the activity of catalases increased until 50 mM in the three explants and diminished after that, the highest values were detected in true leaves. On the other hand, the hypocotyls showed the highest activity of peroxidases with progressive increments until 100 mM of the evaluated salt, while the rest of explants showed a decrease in the enzymatic activities from 75 mM. No appreciable changes were detected in total proteins, while in the carbohydrates there was considerable increase for the highest values of sodium chloride, which could be related to the accumulation of protecting osmolytes.

Keywords : Callus; carbohydrates; enzyme; salinity; Stylosanthes guianensis.

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