Pastos y Forrajes
versão ISSN 0864-0394
WENCOMO, Hilda B et al. Morphoagronomic and isoenzymatic characterization of 23 Leucaena spp. accessions. Pastos y Forrajes [online]. 2011, vol.34, n.4, pp. 413-432. ISSN 0864-0394.
The objective of this work was to evaluate the existing diversity degree in 23 Leucaena accessions, regarding the morphoagronomic and isoenzymatic characteristics. Five species of this genus were included (L. leucocephala, L. lanceolata, L. diversifolia, L. macrophylla and L. esculenta), in addition to the commercial cultivars. The following aspects were evaluated: plant height, number of branches, stem diameter, performance of the phenological flowering and fructification patterns, number of pinnae per leaf and of pinnules per pinna, among others. Besides the electrophoretic analyses peroxidases (Prx),α- and ß- esterases (Est), malate dehydrogenase (Mdh) and alcohol dehydrogenase (Adh) were conducted. The accessions fulfilled the predetermined selection criteria regarding the morphoagronomic characteristics, although not in the same time. In this sense, L. leucocephala cv. Cunningham, cv. Peru, CIAT-9119, CIAT-9438, CIAT-751, CIAT-7988, CIAT-7384, CIAT-7929, CIAT-17480, cv. Ipil-Ipil and cv. CNIA-250; L. lanceolata CIAT-17255 and CIAT-17501, and L. diversifolia CIAT-17270, stood out. The existence of genetic variability within the collection could be detected. The isoenzymatic systems (esterases and peroxidases) turned out to be polymorphic in the studied sample, mainly esterases. On the other hand, the analysis of genetic diversity allowed differentiating L. leucocephala more clearly as compared to the other studied species, although no better discrimination was made within the species.
Palavras-chave : Evaluation; genetic resources; Leucaena spp.