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Pastos y Forrajes

versión impresa ISSN 0864-0394


LEZCANO, J. C; MARTINEZ, B  y  ALONSO, O. Cultural and morphological characterization and identification of ten Fusarium isolates from stored Leucaena leucocephala cv. Peru seeds. Pastos y Forrajes [online]. 2012, vol.35, n.2, pp.187-196. ISSN 0864-0394.

The objective of this work was to characterize cultural and morphologically ten Fusarium isolates, obtained from stored Leucaena leucocephala cv. Peru seeds, aiming at their taxonomic classification. Their pure cultures were preserved in Eppendorf tubes with Potato Dextrose Agar (PDA) medium, in cold chamber at 10ºC. Each isolate was planted in three sterilized Petri dishes, of 9 cm diameter; they contained, separately, the PDA and Potato Sucrose Agar (PSA) media, which constituted the replicates; at their center a mycelial disk was placed, of 7 mm diameter, from 10-15-day-old cultures, also planted in such media. Afterwards, the dishes were sealed with wax paper and incubated for ten days at 25ºC, alternating 8 h of light/16 h of darkness, or in constant darkness. The measured cultural variables were: colony color on the obverse and reverse, texture and lineal and aerial growth; and the morphological variables were: size, shape and color of the vegetative and reproductive structures which appeared in the cultures. A completely randomized design was used, and the data were statistically processed through a descriptive analysis. For the fungi identification different taxonomic keys were used. Isolates 10, 18 and 29 were in correspondence with the characteristics described in scientific literature for the species Fusarium oxysporum Schlecht; 17, 28 and 30, to Fusarium sambucinum Fuckel; 27, to Fusarium semitectum Berk & Rav.; 25 and 26, to Fusarium incarnatum (Rob) Sacc, and 31, to Fusarium chlamydosporum Wollenw & Reinking, which allowed the identification of five Fusarium species associated to the stored seeds.

Palabras clave : Fusarium; identification; Leucaena leucocephala cv. Peru; seeds.

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