SciELO - Scientific Electronic Library Online

 
vol.35 issue3Evaluation of the nutritional potential of four forage plants for feeding rabbit does (Technical note)Evaluation of a probiotic mixture on laying hens feeding in a commercial farm author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

  • Have no cited articlesCited by SciELO

Related links

  • Have no similar articlesSimilars in SciELO

Share


Pastos y Forrajes

Print version ISSN 0864-0394

Abstract

ARECE, J; ROCHE, Yaíma; LOPEZ, Y  and  MOLINA, M. In vitro effect of the aqueous extract of Dichrostachys cinerea (L.) Wight &Arn. on the development of exogenous stages of gastrointestinal strongyles in sheep. Pastos y Forrajes [online]. 2012, vol.35, n.3, pp.301-309. ISSN 0864-0394.

The in vitro effect of aqueous extract of leaves from Dichrostachys cinerea on egg hatching, larval development and migration of third-stage larvae of gastrointestinal strongyles, was evaluated. The treatments were three concentrations of aqueous extracts of D. cinerea leaves (500, 250 and 125 mg/mL), albendazole and levamisole solutions, as well as Phosphate Buffer Saline (PBS) and dimethyl sulfoxide (DMSO) as controls; the design was completely randomized. The hatching percentages showed significant differences among post-treatments. PBS caused the highest hatching rate (96,68%); 30,51% hatched with albendazole and the D. cinerea leaf extracts showed moderate hatching rates (between 49,75 and 66,54%), seemingly dose-dependent. The development of L1/L2 larvae to L3 treated with aqueous D. cinerea extract also showed dose-dependent effects and significant differences with regards to the positive and negative control groups. The dose of 500 mg/mL inhibited larval development in similar percentages as those of albendazole (8,89 and 1,28%, respectively). The PBS medium did not interfere in the larval migration capacity, while with levamisole the values were reduced from 86,45 to 93,92%; the aqueous extracts also significantly reduced such migration, with values between 77,67% and 48,97%. The aqueous D. cinerea extracts showed in vitro anthelmintic activity in the three development stages of the exogenous cycle of gastrointestinal strongyles in sheep; this activity was more evident in the larval development and migration of these nematodes.

Keywords : Dichrostachys cinerea; parasites; sheep.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )