Abstract

ARTEAGA-CHAVEZ, Fátima et al. Selection and identification of Bacillus spp. isolates from the digestive tract of backyard chicken, with probiotic potential. Pastos y Forrajes [online]. 2017, vol.40, n.1, pp.55-64. ISSN 0864-0394.

In order to obtain Bacillus spp. strains with probiotic activity isolates, from the cecum of backyard chicken (Gallus gallus domesticus) were selected and identified. For the isolation the method of seriated dilutions and thermal treatment (71 ºC) was used, for eliminating the cells in vegetative phase and obtaining the endospores. The selection included different tests, such as: resistance to acid pH (2) and to biliary salts (1,5 g.L^-1 of Ox-Bilis), antimicrobial activity against ATCC collection strains and others not belonging to official collections, and resistance to 14 antibiotic types. The data were analyzed according to simple classification design and were processed in the statistical program INFOSTAT. The characterization and identification were developed through biochemical and molecular tests (PCR). Seventy five isolates of Bacillus spp. were obtained from which only two (20 BP and 50 BP) survived the action of the acid pH and of biliary salts, showed antimicrobial activity against pathogen strains and were sensitive to 14 antibiotics. These two isolates, identified as Bacillus subtilis subsp. subtilis, showed potential to be used as probiotics.

Keywords : biliary salts; thermal treatment; viability.

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