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Pastos y Forrajes

Print version ISSN 0864-0394On-line version ISSN 2078-8452


FONTE-CARBALLO, Leydi et al. Quantification of free proline in the diet of the stingless bee livestock of the Matanzas and Mayabeque provinces. Pastos y Forrajes [online]. 2019, vol.42, n.3, pp.218-222. ISSN 0864-0394.

An optimum nutrition through the consumption of a balanced amino acid-rich diet is essential for the good functioning of the immune system of animals. For such reason, the objective of this work was to determine the quantity of free proline, present in the consumed feedstuffs (pollen and honey) by the stingless bee livestock in the Matanzas and Mayabeque provinces. To determine the free proline through spectrophotometry, 60 g of pollen were taken, from sealed amphorae, and 80 mL of honey in each of the ten randomly chosen sampled beehives (five per province). After verifying that the data fulfilled the assumptions of variance homogeneity and normal distribution, a simple variance analysis was performed. The means were compared by Duncan’s test, for a significance level of 5 %. For all the analyses the statistical package Infostat®, version 1.1, was used. The results indicated that, from the statistical point of view, the quantity of free proline found in the honeys from both provinces significantly differed. Meanwhile, the free proline values found in the pollen from Matanzas, were in the range from 4,2 to 19,5 mg/g. The one from Mayabeque showed values from 5,9 to 7,1 mg/g. It is concluded that the honeys, like the pollens, which constitute the feeding source of the stingless bee livestock of both provinces, have an adequate proportion of free proline. Thus, the livestock located in both zones has the necessary amino acid support in its diet to develop adequate immune response to any microbial agent.

Keywords : amino acids; honey; pollen.

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