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Revista Cubana de Oftalmología

Print version ISSN 0864-2176On-line version ISSN 1561-3070

Abstract

GUERRA ALMAGUER, Michel et al. Concordance between different cell counts according to morphologic and morphometric parameters of the corneal endothelium. Rev Cubana Oftalmol [online]. 2019, vol.32, n.4, e767.  Epub Feb 15, 2020. ISSN 0864-2176.

Objective:

To estimate the morphological and morphometric values ​​of the corneal endothelium according to the number of cells and evaluate the interobserver concordance for the different parameters, estimated according to the different cell counts in adults without corneal alterations.

Methods:

An observational, descriptive and cross-sectional case series research was carried out in the Refractive Surgery Service of Ramón Pando Ferrer Cuban Institute of Ophthalmology during two years of study. After applying the exclusion criteria, the sample was made up of 200 eyes of 100 adult patients without corneal alterations. Non-contact endothelial microscopy SP-3000P was performed to identify morphological values (hexagonality and coefficient of variability) and morphometric values (cell density), as well as the average corneal cell size according to the number of cells evaluated.

Results:

According to the amount of endothelial cells evaluated, there were no significant differences between morphological and morphometric variables (p>0.05) in both eyes. The agreement between the different cell counts according to the values ​​of the interclass correlation coefficients (ICC) were all high. The interobserver concordance and ICCs were also high, except for hexagonality.

Conclusions:

The morphological and morphometric values ​​of the corneal endothelium, according to the number of cells evaluated, are similar in all cell counts. Good concordance between the different cell counts studied for the different estimated parameters is demonstrated.

Keywords : Concordance; interclass correlation coefficient; corneal endothelium; specular microscopy.

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