Resumen

KELY MARTINO ZAGOVALOV, Tamara et al. Determination of Listeria spp in cheese and sausages sold in Cuba. Rev Cubana Salud Pública [online]. 2005, vol.31, n.3. ISSN 0864-3466.

Listeria monocytogenes is an emerging pathogen causing a food-borne disease called listeriosis that seriously affects people and generally leaves sequelae in survivors. Detection of L. monocytogenes in high-risk foodstuffs such as ready-to-eat food is overly important. The present paper determined the incidence of Listeria in 54 cheese samples and 98 samples of sausage and smoked products that are sold in Cuba by using a traditional method for qualitative and quantitative detection of L. monocytogenes according to Standard UNE-EN ISO 11290-2 (1997) and Draft International Standard ISO/DIS 11290-2 (1995). Additional biochemical tests for detection of other Listeria species. A rapid diagnostic test was used for qualitative detection. L. monocytogenes was isolated from blue cheese, salami and mortadella samples. L innocua was detected in Frescal and Cubanito types of cheese, chorizo (highly seasoned pork sausage), longaniza magro (spiced pork sausage) and blood sausage whereas L. weishimeri was found in sausages. Two L. Species were present in the same salami sample. In other three samples (smoked chicken, chorizo and mortadella) the rapid test could detect Listeria although it was not possible to isolate it by the traditional method, which is due to the higher sensitivity of the former method. Enumeration was 102 UFC/g of food in all cases. The qualitative technique allowed demonstrating the presence of L. monocytogenes in three of the analyzed samples, which restricts the sale and consumption of the above-mentioned products

Palabras clave : Listeria monocytogenes; Listeria; cheese; pork sausages; smoked products; microbiological determination; food poisoning.

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