Revista de Protección Vegetal
versión ISSN 1010-2752
RODRIGUEZ-ROMERO, A; POSOS PONCE, P; PETEIRA, Belkis y SURIS, Moraima. EVALUATION OF THREE DNA EXTRACTION PROTOCOL FOR INSECTS FROM THE ORDER THYSANOPTERA. Rev. Protección Veg. [online]. 2011, vol.26, n.3, pp. 187-190. ISSN 1010-2752.
Three protocols were tested to evaluate the efficiency of the genomic DNA extraction from insects of the Order Thysanoptera. Ethanol preserved adults of the Genera Frankiniella Karny and Thrips Linneaus were used as the material for the extraction. The efficiency was measured through the amplification of the ITS region in a multiplex PCR assay using the primers ITS1, ITS2, CS249 and CS250. The PCR product was separated by electrophoresis on 1% agarose gel. It was demonstrated that no amplification was obtained following the protocol of Aljanabi and Martínez, whereas the protocols described by Rugman and Moritz showed well defined band patterns, suggesting that both procedures can be used for the DNA extractions.
Palabras llave : Thysanoptera; DNA extraction.