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Revista de Protección Vegetal
versão impressa ISSN 1010-2752
Resumo
SANCHEZ, Yaíma; CORREA, Teresa M; ABREU, Yudith e PINO, Oriela. Effect of the essential oil of Piper auritum Kunth and its components against Xanthomonas albilineans (Ashby) Dowson and Xanthomonas campestris pv campestris (Pammel) Dowson. Rev. Protección Veg. [online]. 2013, vol.28, n.3, pp.204-210. ISSN 1010-2752.
The aim of this study was to identify the component (s) of the essential oil of Piper auritum associated with the antibacterial effect against Xanthomonas albilineans and Xanthomonas campestris pv. campestris. The essential oil of P. auritum was obtained by hydrodistillation in a Clevenger type apparatus. The Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) against both bacteria were determined by the method of serial dilutions. The components responsible for the biological activity were identified by fractionating the essential oil by dry chromatography, and the fractions were investigated by GC / MS and tested against the above mentioned bacteria. This oil has a strong antibacterial activity with MIC and MBC of 0,12 and 0,25 mg.ml-1 against X. albilineans and X. campestris pv. campestris, respectively. The principal component responsible for the observed biological activity was safrole; however, the whole essential oil met all the requirements for being used as a promissory antibacterial agent.
Palavras-chave : Xanthomonas campestris pv. campestris; productos naturales; essential oil; Piper auritum; Xanthomonas albilineans; Xanthomonas campestris pv. campestris; natural products.