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Revista de Protección Vegetal

versión impresa ISSN 1010-2752versión On-line ISSN 2224-4697

Resumen

SALVADOR PERNIOLA, Omar et al. Biofumigation with Brassica juncea L. Czerniak and Sinapis alba L. In vitro action on the growth of Trichoderma spp. and Azospirillum brasilense Tarrand, Krieg et Döbereiner. Rev. Protección Veg. [online]. 2016, vol.31, n.1, pp.57-62. ISSN 1010-2752.

The aim of this work was to evaluate the in vitro effect of the biofumigation with Brassica juncea L. Czerniak and Sinapis alba L. on the growth of Trichoderma spp. and Azospirillum brasilense Tarrand, Krieg et Döbereiner. Five doses (5, 15, 25, 35 and 55 g) of triturated plant material from B. juncea and S. alba were placed separately in plastic containers. Petri dishes with culture medium inoculated with Trichoderma spp. or A. brasilense, were placed on top of the plant material. The plastic containers were then covered and incubated in darkness for 7 days at 25±2ºC (Trichoderma spp.) and 31±2ºC (A. brasilense). After that, the diameter of the colonies of Trichoderma spp. was measured, and the number of colony forming units of A. brasilense was counted. The results indicated that: i) fungistatic effect of B. juncea and S. alba was not observed on Trichoderma spp. at any doses; ii) B. juncea inhibited colony growth of A. brasilense at doses of 15 g or higher, with an increasing inhibition as the biofumigant dose increased; iii) S. alba did not inhibit colony growth of A. brasilense at any doses. These in vitro results suggest that the technique of biocontrol with the antagonist fungus Trichoderma spp. can be compatible with biofumigation with B. juncea and S. alba. Also, A. brasilense can only be compatible with biofumigation with S. alba and with low doses of B. juncea.

Palabras clave : antagonistic fungi; rhizobacteria (PGPR); Brassicaceae; biocontrol.

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