Abstract

SARTI, Gabriela C.; CRISTOBAL MIGUEZ, Ana E.J.  and  CURA, Alfredo J.. Optimization of the culture conditions for the development of a bacterial biofilm and its application as a biofertilizer in Solanum lycopersicum L. var. Río grande. Rev. Protección Veg. [online]. 2019, vol.34, n.2, e01.  Epub Aug 01, 2019. ISSN 1010-2752.

The aim of this work was to optimize the growth conditions of Bacillus subtilis subsp. spizizenii for the development of a biofilm and its use as a biofertilizer in tomato seeds (Solanum lycopersicum L.var. Río Grande). Different culture media were tested for the development of biofilms, which were dried and weighed to determine their mass. The time for biofilm disintegration was also determined. The bacteria were introduced under two treatments: fertile or tindalized soil. The aerial and root dry mass was weighed. The differences in the biomass of the aerial part and root of the tomato plants were analyzed by a simple ANOVA. The means were compared by the Tukey test at a significance level of 0.05. The statistical analysis was carried out with the software Infostat 2016. The biofilms obtained were evaluated in terms of arithmetic percentages and standard deviations of three repetitions. The greatest amount of biofilm was obtained in the glucose potato medium, and it was the most resistant to disintegration. No higher seedling growth was shown by the treatment with inoculated seeds in fertile soil compared with the uninoculated controls. However, when the tindalized soil was used, the aerial and root biomass of the inoculated seedlings was significantly higher than in the uninoculated controls. The biofilm developed by Bacillus in the potato glucose medium could be used as a biofertilizer in tomato.

Keywords : Bacillus subtilis; biofertilizers; biofilm; Solanum lycopersicum L; tomato.

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