Abstract

MENA LOPEZ, Lázaro; NUNEZ MESA, Ciro; PEREZ CABARCO, Nancy  and  FERNANDEZ ESTRADA, José. Influence of temperature, time of conservation, and absolute value of serum samples in quantification results of tetanus antitoxin. AMC [online]. 2002, vol.6, n.4, pp.405-413. ISSN 1025-0255.

Precision and accuracy of UMELISA kit in IgG tetanus antitoxin dosification was assessed. Concentration variability of antibodies was studied according to time and storage temperature in 34 special donors of tetanus antitoxin hyperimmune plasma immunized with tetanus antitoxin vaccine of national production and was subjected to procedures of donation for automated plasmapheresis. Two study group were chosen as to serum immunoglobulin concentration (<10 UI/ml and >10 UI/ml) and were worked with preserved samples at -30 ºC and of 2-8 ºC. Statistic test used in the processing of information showed that the technique was precise and accurate and that greater fluctuations occur when titles are low and the conservation is at 2-8 ºC.

Keywords : TETANUS ANTITOXIN; QUANTITATIVE ANALYSIS; BLOOD PRESERVATION.

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