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Revista Archivo Médico de Camagüey
versión On-line ISSN 1025-0255
Resumen
FALCON ALMEIDA, Yadira et al. Stability of a control serum for total proteins used as a biochemical controller in clinical laboratories. AMC [online]. 2014, vol.18, n.4, pp. 401-414. ISSN 1025-0255.
Background: one of the main ways to guarantee the quality in clinical labs is by using a control serum or controller. A problem we face is the lack of these products in the domestic market due to their high cost. Objective: to evaluate the stability of an adult bovine serum for total proteins as a biochemical controller in clinical labs. Method: a quasiexperiment was conducted during three years (January 2010-December 2012) through a probabilistic sampling. The stability in real time of the serum enriched with total proteins was evaluated during 12 months under two temperatures (refrigeration and freezing). The study was designed for three batches per month and three determinations per batch, for a total number of sample of the study (N=63). Results: a liquid control serum for total proteins was obtained. The concentration of total proteins in the product obtained under refrigeration and freezing conditions kept stable for 12 months. The obtained product, when compared to the control serum from the company OLYMPUS, showed similar fluctuations regarding the concentration of total proteins. Conclusions: a reference material was obtained as a stable controller for a period of 12 months.
Palabras clave : SERUM [chemistry]; REFERENCE STANDARDS; SEQUENCE ANALYSIS, PROTEIN; QUALITY CONTROL; EPIDEMIOLOGY EXPERIMENTAL.