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Vaccimonitor

Print version ISSN 1025-028XOn-line version ISSN 1025-0298

Abstract

OCHOA, Rolando et al. Guide for the standardization of enzyme immunoassays for use in vaccine trials. Vaccimonitor [online]. 2000, vol.9, n.3, pp.13-18. ISSN 1025-028X.

The standardization of enzyme immunoassays, for use in evaluation of pre-clinical and clinical vaccine trials, was reviewed. A practical guide was made. The immunoassay principles and the assay optimization step - including the preparation of standards and controls - are analyzed. Our experience is presented.

Keywords : Standardization; enzyme immunoassays; ELISA; vaccines.

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