Abstract

GUTIERREZ, Beatriz et al. First report on the isolation of Helicobacter pylori associated to digestive diseases in the City of Havana. Vaccimonitor [online]. 2001, vol.10, n.1, pp.22-26. ISSN 1025-028X.

Infection by Helicobacter pylori plays an important role in different digestive diseases. At present it is accepted that this microorganism is related to active chronic gastritis, peptic ulcer (gastric as well as duodenal), gastric adenocarcinoma and B-cell gastric lymphoma of the lymphoid tissue associated with mucosa (MALT lymphoma). The study included 121 patients: 53 having an endoscopic diagnosis of gastritis, 21 with peptic ulcer, 20 with duodenal ulcers, 3 adenocarcinomas of the gastric antro, 5 esophagus neoplasm, and 19 normal panendoscopies (dyspeptic). Gastroscopy was carried out on all patients and four biopsy fragments were taken from the antro and body regions of the stomach. For diagnosis and isolation of H. pylori a fast urease test was used. Gram stain and culture in brain heart agar with 10% horse serum, 0,25% yeast extract and Dent supplement (OXOID SR 147) during 7 days at 37 ºC in a jar with a microaerophyllic environment were also done. The presence of catalase and oxidase were also evaluated. An infection prevalence of 79,2% was observed in chronic gastritis patients, 100% in duodenal ulcer patients, and 90,4% in those with gastric ulcer, in agreement with the reports from other authors. In 100% of the gastric adenocarcinoma cases and 60% of the ones with esophagus neoplasm the microorganism was isolated. Isolation of H pylori in 78,9% of the endoscopic cases considered and reported as normal is a warning of the prevalence in dyspeptic patients.

Keywords : H. pylori; prevalence; endoscopic diagnosis; isolations; warning.

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