Abstract

GONZALEZ, Marta et al. Study of the Passive Protection induced by the serum of vaccinated people with vax-SPIRAL (Trivalent antileptospirosic vaccine) in Hámsters challenged with virulents strains of Leptospira canicola, copenhageni y mozdok. Vaccimonitor [online]. 2001, vol.10, n.3, pp.1-6. ISSN 1025-028X.

As part of the characterisation studies of the human immune response induced in vaccinated people with vax-SPIRAL (Trivalent Antileptospirosic Vaccine), 109 and 69 voluntary people from the Universidad Agropecuaria and Centro Nacional de Sanidad Agropecuaria, Havana Province were vaccinated respectively. Two blood samples were taken, before vaccination (T0) and 21 days after the second dose (T1). The specific IgG response was measured by means a quantitative indirect ELISA and its protective effect was evaluated by a passive protection test in hamsters. Taking into consideration the ELISA results four pools of sera were prepared and inoculated into the hamsters. They were challenge 18-24 hours later with 1000 -10 000 DL50 of virulent strains of serovars canicola, copenhageni and mozdok. The animals were observed during 14 days. The correlation coefficient of 0.94 and 0.97 indicating a relatively strong relationship between the IgG level and protection for canicola and mozdok respectively, for copenhageni a correlation coefficient equals to 0,83 was obtained indicating a moderately strong relationship. The specific IgG response of sera from risk people was related with protection.

Keywords : Leptospirosis; vaccination; passive protection; humoral response; Leptospira canicola; Leptospira copenhageni; Leptospira mozdok.

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